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Company Abstracts :: 1994 :: Selected Company Abstract
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LJP 249: A NEW OLIGONUCLEOTIDE
CONJUGATE FOR THE SUPPRESSION OF ANTI-DOUBLE STRANDED DNA ANTIBODIES
David S. Jones, John P. Hachmann, Stephen A. Osgood, Stephen M.
Coutts
Conjugates of 5’-modified single stranded (ss) and double-stranded
(ds) oligonucleotides (ON), with derivatized polyethylene glycol
(PEG) and with keyhole limpet hemocyanin (KLH), were synthesized.
A 5’ thiol containing ON was synthesized from a new phosphoramidite
linker and was reacted with PEG, derivatized with four iodoacetyl
groups, to provide a conjugate of four ss-ON per PEG. Complementary
ON strands were annealed to provide LJP 249, an anti-DNA suppressive
conjugate of four ds-ON per PEG. A 5’ vicinal diol containing-ON
was synthesized from another new phosphoramidite linker, and was
used to prepare a conjugate of about five ds-ON per KLH for use
as an immunogen. The complete chemical syntheses of both conjugates
are described.
Presented at the
207th meeting of the
American Chemical Society
Spring, 1994, San Diego, CA.
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