LJP-249 SUPPRESSES ANTI-DS OLIGONUCLEOTIDE ANTIBODIES AND PLAQUE FORMING CELLS IN IMMUNIZED MICE.

G. Michael Iverson, Jacqueline F. Crisologo, and Stephen M. Coutts.

La Jolla Pharmaceutical Company, San Diego, CA 92121

LJP-249, a new conjugate of synthetic, double stranded oligonucleotides (dsON) and polyethylene glycol, was used to suppress antibody responses in mice immunized with a conjugate dsON and keyhole limpet hemocyanin (KLH). In this model of SLE, mice were primed with dsON-KLH adsorbed on alum and pertussis; after at least three weeks, they were dosed ip with LJP-249 or saline. After one week, the mice were boosted with dsON-KLH in saline. A week later, the mice were bled and anti-ON antibodies were determined by Farr assay. Antibody forming cells were determined by plaque assay after making single cell suspensions from the spleens of mice 4 days after boosting. LJP-249 significantly suppressed both anti-dsON and dsON-plaque forming cells in a dose dependent manner.

Presented at
The 207th meeting of the American Chemical Society
Spring, 1994, San Diego, CA.